Coding
Part:BBa_K1957009
Designed by: Nancy Teng Group: iGEM16_NRP-UEA-Norwich (2016-10-13)
FeFe Hydrogenase
Entire FeFe Hydrogenase cluster ligated together and inserted into pSB1C3. This cluster has a Strep-II tag on the N-terminus, thus is made out of the following BioBricks: Bba_K1957001, Bba_K1957003 and Bba_K1957004.
From the sequencing data, it was confirmed that the HydABC cluster was excised out of pBAD and ligated into pSB1C3 successfully (Fig.1) The 'red' section towards the end of the sequence is the primer sequence which was not included in the HydABC sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1208
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Categories
Parameters
None |