Coding

Part:BBa_K1957009

Designed by: Nancy Teng   Group: iGEM16_NRP-UEA-Norwich   (2016-10-13)


FeFe Hydrogenase

Entire FeFe Hydrogenase cluster ligated together and inserted into pSB1C3. This cluster has a Strep-II tag on the N-terminus, thus is made out of the following BioBricks: Bba_K1957001, Bba_K1957003 and Bba_K1957004.

Figure 1: Screenshot of sequencing data of colony aligned with HydABC using Benchling. Consensus alignment is shown, grey indicates 100% alignment whereas areas indicated in red are did not align.


From the sequencing data, it was confirmed that the HydABC cluster was excised out of pBAD and ligated into pSB1C3 successfully (Fig.1) The 'red' section towards the end of the sequence is the primer sequence which was not included in the HydABC sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1208


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